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mouse monoclonal anti psd95 antibody  (Novus Biologicals)


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    Novus Biologicals mouse monoclonal anti psd95 antibody
    a , Immunoblot analysis of PARIS, pSer202/pThr205-tau (AT8), pThr181-tau (AT270), pSer396-tau, total tau, and β-actin in entorhinal cortex samples from 10-month-old mice. The location of PARIS is indicated by arrows. b–e , Quantification of total tau, AT8, AT270, and pSer396-tau levels from a . Data are mean ± SEM (PS19, n = 8; PS19; Paris -/- , n = 5). Statistical significance was assessed by two-tailed t -test. f , Immunoblot analysis of PARIS, pSer202/pThr205-tau (AT8), pThr181-tau (AT270), pSer396-tau, total tau, and β-actin in frontal cortex samples from 10-month-old mice. The location of PARIS is indicated by arrows. g–j , Quantification of total tau, AT8, AT270, and pSer396-tau levels from f . Data are mean ± SEM (PS19, n = 8; PS19; Paris -/- , n = 5). Statistical significance was assessed by two-tailed t -test. k , Representative immunofluorescence images of SYNAPSIN I (green), <t>PSD95</t> (red), and DAPI (blue) in the hippocampus of 10-month-old mice. Scale bar, 50 µm. l , m , Quantification of SYNAPSIN I–positive area and PSD95–positive area from k . Data are mean ± SEM (WT, n = 29; PS19, n = 31; PS19; Paris -/- , n = 29; images from 5–8 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. Comparisons with P ≤ 0.05 are marked on the graph.
    Mouse Monoclonal Anti Psd95 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti-+psd-+95/PSD-95+Antibody+(6G6-1C9)+-+BSA+Free/bio_rxiv__64898__2025__12__06__692741-228-18-22
    Average 94 stars, based on 33 article reviews
    mouse monoclonal anti psd95 antibody - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Neuronal PARIS-STAT3 axis drives tau pathology and glial activation in Alzheimer’s disease"

    Article Title: Neuronal PARIS-STAT3 axis drives tau pathology and glial activation in Alzheimer’s disease

    Journal: bioRxiv

    doi: 10.64898/2025.12.06.692741

    a , Immunoblot analysis of PARIS, pSer202/pThr205-tau (AT8), pThr181-tau (AT270), pSer396-tau, total tau, and β-actin in entorhinal cortex samples from 10-month-old mice. The location of PARIS is indicated by arrows. b–e , Quantification of total tau, AT8, AT270, and pSer396-tau levels from a . Data are mean ± SEM (PS19, n = 8; PS19; Paris -/- , n = 5). Statistical significance was assessed by two-tailed t -test. f , Immunoblot analysis of PARIS, pSer202/pThr205-tau (AT8), pThr181-tau (AT270), pSer396-tau, total tau, and β-actin in frontal cortex samples from 10-month-old mice. The location of PARIS is indicated by arrows. g–j , Quantification of total tau, AT8, AT270, and pSer396-tau levels from f . Data are mean ± SEM (PS19, n = 8; PS19; Paris -/- , n = 5). Statistical significance was assessed by two-tailed t -test. k , Representative immunofluorescence images of SYNAPSIN I (green), PSD95 (red), and DAPI (blue) in the hippocampus of 10-month-old mice. Scale bar, 50 µm. l , m , Quantification of SYNAPSIN I–positive area and PSD95–positive area from k . Data are mean ± SEM (WT, n = 29; PS19, n = 31; PS19; Paris -/- , n = 29; images from 5–8 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. Comparisons with P ≤ 0.05 are marked on the graph.
    Figure Legend Snippet: a , Immunoblot analysis of PARIS, pSer202/pThr205-tau (AT8), pThr181-tau (AT270), pSer396-tau, total tau, and β-actin in entorhinal cortex samples from 10-month-old mice. The location of PARIS is indicated by arrows. b–e , Quantification of total tau, AT8, AT270, and pSer396-tau levels from a . Data are mean ± SEM (PS19, n = 8; PS19; Paris -/- , n = 5). Statistical significance was assessed by two-tailed t -test. f , Immunoblot analysis of PARIS, pSer202/pThr205-tau (AT8), pThr181-tau (AT270), pSer396-tau, total tau, and β-actin in frontal cortex samples from 10-month-old mice. The location of PARIS is indicated by arrows. g–j , Quantification of total tau, AT8, AT270, and pSer396-tau levels from f . Data are mean ± SEM (PS19, n = 8; PS19; Paris -/- , n = 5). Statistical significance was assessed by two-tailed t -test. k , Representative immunofluorescence images of SYNAPSIN I (green), PSD95 (red), and DAPI (blue) in the hippocampus of 10-month-old mice. Scale bar, 50 µm. l , m , Quantification of SYNAPSIN I–positive area and PSD95–positive area from k . Data are mean ± SEM (WT, n = 29; PS19, n = 31; PS19; Paris -/- , n = 29; images from 5–8 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. Comparisons with P ≤ 0.05 are marked on the graph.

    Techniques Used: Western Blot, Two Tailed Test, Immunofluorescence

    a , Percentage of time in the novel arm, total distance traveled, and arm entries in the first Y-maze test. Data are mean ± SEM (Control, n = 9; CamK-PARIS, n = 9; CamK-PARIS (+Napabucasin), n = 10). Group differences were assessed by one-way ANOVA followed by Tukey’s post hoc test. b , Mean speed during Barnes maze training. Data are mean ± SEM (Control, n = 9; CamK-PARIS, n = 8; CamK-PARIS (+Napabucasin), n = 8). Group differences were assessed by one-way ANOVA followed by Tukey’s post hoc test. c , Representative immunofluorescence images of SYNAPSIN I (green), PSD95 (red), and DAPI (blue) in the hippocampus of mice. Scale bar, 50 µm. d , e , Quantification of SYNAPSIN I–positive area and PSD95–positive area from c . Data are mean ± SEM (Control, n = 33; CamK-PARIS, n = 32; CamK-PARIS (+Napabucasin), n = 31; images from 5–8 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. Comparisons with P ≤ 0.05 are marked on the graph. f , Representative co-immunostaining images of GFAP (red), C3 (green), and DAPI (blue) in the hippocampus of control and CamK-PARIS mice. Scale bar, 50 µm. g–i , Quantification of GFAP and relative C3 fluorescence from f . Data are mean ± SEM (Control, n = 38; CamK-PARIS, n = 47; CamK-PARIS (+Napabucasin), n = 51; images from 7–12 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. j , Representative co-immunostaining images of IBA1 (green), CD16/CD32 (red), and DAPI (blue) in the hippocampus of control and CamK-PARIS mice. Scale bar, 50 µm. k , l , Quantification of IBA1 and relative CD16/CD32 fluorescence from j . Data are mean ± SEM (Control, n = 43; CamK-PARIS, n = 45; CamK-PARIS (+Napabucasin), n = 50; images from 7–10 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. Comparisons with P ≤ 0.05 are marked on the graph.
    Figure Legend Snippet: a , Percentage of time in the novel arm, total distance traveled, and arm entries in the first Y-maze test. Data are mean ± SEM (Control, n = 9; CamK-PARIS, n = 9; CamK-PARIS (+Napabucasin), n = 10). Group differences were assessed by one-way ANOVA followed by Tukey’s post hoc test. b , Mean speed during Barnes maze training. Data are mean ± SEM (Control, n = 9; CamK-PARIS, n = 8; CamK-PARIS (+Napabucasin), n = 8). Group differences were assessed by one-way ANOVA followed by Tukey’s post hoc test. c , Representative immunofluorescence images of SYNAPSIN I (green), PSD95 (red), and DAPI (blue) in the hippocampus of mice. Scale bar, 50 µm. d , e , Quantification of SYNAPSIN I–positive area and PSD95–positive area from c . Data are mean ± SEM (Control, n = 33; CamK-PARIS, n = 32; CamK-PARIS (+Napabucasin), n = 31; images from 5–8 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. Comparisons with P ≤ 0.05 are marked on the graph. f , Representative co-immunostaining images of GFAP (red), C3 (green), and DAPI (blue) in the hippocampus of control and CamK-PARIS mice. Scale bar, 50 µm. g–i , Quantification of GFAP and relative C3 fluorescence from f . Data are mean ± SEM (Control, n = 38; CamK-PARIS, n = 47; CamK-PARIS (+Napabucasin), n = 51; images from 7–12 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. j , Representative co-immunostaining images of IBA1 (green), CD16/CD32 (red), and DAPI (blue) in the hippocampus of control and CamK-PARIS mice. Scale bar, 50 µm. k , l , Quantification of IBA1 and relative CD16/CD32 fluorescence from j . Data are mean ± SEM (Control, n = 43; CamK-PARIS, n = 45; CamK-PARIS (+Napabucasin), n = 50; images from 7–10 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. Comparisons with P ≤ 0.05 are marked on the graph.

    Techniques Used: Control, Immunofluorescence, Immunostaining, Fluorescence

    Related Articles

    Saline:

    Article Title: A sensory and motor neuropathy caused by a genetic variant of NAMPT
    Article Snippet: .. The membranes were blocked by 5% (w/v) BSA for 1 hour in tris-buffered saline containing 0.1% (v/v) Tween 20 and incubated with the following primary antibodies: rabbit anti-NAMPT polyclonal antibody (1:500, A300-372A, Bethyl Laboratories, TX, USA; RRID:AB_2251232), goat anti-NMANT3 (1:1000, ab121030, Abcam, MA, USA; RRID:AB_10903780), rabbit anti-Sirt1 antibody (07-131, 1:1000, Millipore; RRID:AB_11214517), rabbit anti-Sirt3 antibody (1:1000, ab86671, Abcam; RRID:AB_10861832), rabbit anti-Mfn2 antibody (1:1000, no. 9482, Cell Signaling; RRID:AB_2716838), rabbit anti-Drp1 antibody (1:1000, ABT155, Millipore; RRID:AB_11203145), rabbit anti-p-Drp1 antibody (1:1000, no. 3455, Cell Signaling; RRID:AB_2085352), rabbit anti-acetylated lysine antibody (1:1000, no. 9441, Cell Signaling; RRID:AB_331805), rabbit anti-TOM20 antibody (1:1000, sc-11415, Santa Cruz Biotechnology; RRID:AB_2207533), rabbit anti-PGC-1α polyclonal antibody (1:500, sc-13067, Santa Cruz Biotechnology; RRID:AB_2166218), rabbit anti-NRF-1 polyclonal antibody (1:500, sc-33771, Santa Cruz Biotechnology; RRID:AB_2153203), mouse anti-synaptophysin (1:1000, S-5768, Sigma-Aldrich; RRID:AB_477523), mouse anti-vGluT1 (1:1000, NBP2-59329, Novus Biologicals), rabbit anti-NMDAR2A (1:1000, NB300-105, Novus Biologicals; RRID:AB_10001400), rabbit anti-NMDAR2B (1:1000, NB300-106, Novus Biologicals; RRID:AB_10000537), mouse anti-GluA1 (1:1000, NBP2-22399, Novus Biologicals), rabbit anti-GluA2 (1:1000, NBP2-75510, Novus Biologicals), mouse anti-PSD95 (1:1000, NB300-556 Biologicals; RRID:AB_2092366), mouse anti-β-actin (1:2000, a3853, Sigma-Aldrich; RRID:AB_262137). .. The membranes were then incubated with second antibodies including rabbit anti-mouse immunoglobulin G (IgG)–peroxidase (A9044, Sigma-Aldrich; RRID:AB_258431) or goat anti-rabbit IgG-peroxidase (A0545, Sigma-Aldrich; RRID:AB_257896).

    Article Title: Low-dose dietary vorinostat increases brain histone acetylation levels and reduces oxidative stress in an Alzheimer's disease mouse model.
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    Article Title: Low-dose dietary vorinostat increases brain histone acetylation levels and reduces oxidative stress in an Alzheimer's disease mouse model
    Article Snippet: Nonspecific binding was blocked using 5% Blotto nonfat dry milk (Cat# sc-2325, Santa Cruz Biotechnology, Dallas, TX, USA) with 0.1%Tween-20 for 1.5 h at room temperature (RT). .. Membranes were probed with rabbit monoclonal anti-acetyl-Histone H3 (Lys9, C5B11, Cat# 9649, Cell Signaling Technology, Danvers, MA, USA) diluted 1:1000 in 5% BSA in Tris-buffered saline with 0.1%Tween-20 (TBST; 20 mM Tris·HCl (pH 7.6), 137 mM NaCl, and 0.1% (vol/vol) Tween-20), rabbit monoclonal anti-Histone H3 (D1H2, Cat# 4499, Cell Signaling Technology) diluted 1:2000, mouse monoclonal anti-synaptophysin (SY38, Cat# ab8049, Abcam, Cambridge, UK) diluted 1:700, mouse monoclonal anti-PSD-95 (6G6-1C9, Cat# NB300-556, Novus Biologicals, Centennial, CO, USA) diluted 1:1000, in 5% nonfat dry milk in TBST, and incubated overnight at 4°C using gentle shaking. .. Membranes were washed with TBST 3x 5 min each and incubated with horseradish peroxidase-conjugated anti-rabbit IgG (1:2000 dilution, Cat# 7074, Cell Signaling Technology) and anti-mouse IgG (1:2000, Novus Biologicals), for 1 h at RT in 5% non-fat dry milk in TBST.

    Incubation:

    Article Title: A sensory and motor neuropathy caused by a genetic variant of NAMPT
    Article Snippet: .. The membranes were blocked by 5% (w/v) BSA for 1 hour in tris-buffered saline containing 0.1% (v/v) Tween 20 and incubated with the following primary antibodies: rabbit anti-NAMPT polyclonal antibody (1:500, A300-372A, Bethyl Laboratories, TX, USA; RRID:AB_2251232), goat anti-NMANT3 (1:1000, ab121030, Abcam, MA, USA; RRID:AB_10903780), rabbit anti-Sirt1 antibody (07-131, 1:1000, Millipore; RRID:AB_11214517), rabbit anti-Sirt3 antibody (1:1000, ab86671, Abcam; RRID:AB_10861832), rabbit anti-Mfn2 antibody (1:1000, no. 9482, Cell Signaling; RRID:AB_2716838), rabbit anti-Drp1 antibody (1:1000, ABT155, Millipore; RRID:AB_11203145), rabbit anti-p-Drp1 antibody (1:1000, no. 3455, Cell Signaling; RRID:AB_2085352), rabbit anti-acetylated lysine antibody (1:1000, no. 9441, Cell Signaling; RRID:AB_331805), rabbit anti-TOM20 antibody (1:1000, sc-11415, Santa Cruz Biotechnology; RRID:AB_2207533), rabbit anti-PGC-1α polyclonal antibody (1:500, sc-13067, Santa Cruz Biotechnology; RRID:AB_2166218), rabbit anti-NRF-1 polyclonal antibody (1:500, sc-33771, Santa Cruz Biotechnology; RRID:AB_2153203), mouse anti-synaptophysin (1:1000, S-5768, Sigma-Aldrich; RRID:AB_477523), mouse anti-vGluT1 (1:1000, NBP2-59329, Novus Biologicals), rabbit anti-NMDAR2A (1:1000, NB300-105, Novus Biologicals; RRID:AB_10001400), rabbit anti-NMDAR2B (1:1000, NB300-106, Novus Biologicals; RRID:AB_10000537), mouse anti-GluA1 (1:1000, NBP2-22399, Novus Biologicals), rabbit anti-GluA2 (1:1000, NBP2-75510, Novus Biologicals), mouse anti-PSD95 (1:1000, NB300-556 Biologicals; RRID:AB_2092366), mouse anti-β-actin (1:2000, a3853, Sigma-Aldrich; RRID:AB_262137). .. The membranes were then incubated with second antibodies including rabbit anti-mouse immunoglobulin G (IgG)–peroxidase (A9044, Sigma-Aldrich; RRID:AB_258431) or goat anti-rabbit IgG-peroxidase (A0545, Sigma-Aldrich; RRID:AB_257896).

    Article Title: Low-dose dietary vorinostat increases brain histone acetylation levels and reduces oxidative stress in an Alzheimer's disease mouse model.
    Article Snippet: Nonspecific binding was blocked using 5% Blotto nonfat dry milk (Cat# sc-2325, Santa Cruz Biotechnology, Dallas, TX, USA) with 0.1% Tween-20 for 1.5 h at room temperature (RT). .. Membranes were probed with rabbit monoclonal anti-acetyl-Histone H3 (Lys9, C5B11, Cat# 9649, Cell Signaling Technology, Danvers, MA, USA) diluted 1:1000 in 5% BSA in Tris-buffered saline with 0.1% Tween-20 (TBST; 20 mM Tris·HCl (pH 7.6), 137 mM NaCl, and 0.1% (vol/vol) Tween-20), rabbit monoclonal anti-Histone H3 (D1H2, Cat# 4499, Cell Signaling Technology) diluted 1:2000, mouse monoclonal antisynaptophysin (SY38, Cat# ab8049, Abcam, Cambridge, UK) diluted 1:700, mouse monoclonal anti-PSD-95 (6G6-1C9, Cat# NB300-556, Novus Biologicals, Centennial, CO, USA) diluted 1:1000, in 5% nonfat dry milk in TBST, and incubated overnight at 4°C using gentle shaking. .. Membranes were washed with TBST 3x 5 min each and incubated with horseradish peroxidase-conjugated anti-rabbit IgG (1:2000 dilution, Cat# 7074, Cell Signaling Technology) and anti-mouse IgG (1:2000, Novus Biologicals), for 1 h at RT in 5% non-fat dry milk in TBST.

    Article Title: Low-dose dietary vorinostat increases brain histone acetylation levels and reduces oxidative stress in an Alzheimer's disease mouse model
    Article Snippet: Nonspecific binding was blocked using 5% Blotto nonfat dry milk (Cat# sc-2325, Santa Cruz Biotechnology, Dallas, TX, USA) with 0.1%Tween-20 for 1.5 h at room temperature (RT). .. Membranes were probed with rabbit monoclonal anti-acetyl-Histone H3 (Lys9, C5B11, Cat# 9649, Cell Signaling Technology, Danvers, MA, USA) diluted 1:1000 in 5% BSA in Tris-buffered saline with 0.1%Tween-20 (TBST; 20 mM Tris·HCl (pH 7.6), 137 mM NaCl, and 0.1% (vol/vol) Tween-20), rabbit monoclonal anti-Histone H3 (D1H2, Cat# 4499, Cell Signaling Technology) diluted 1:2000, mouse monoclonal anti-synaptophysin (SY38, Cat# ab8049, Abcam, Cambridge, UK) diluted 1:700, mouse monoclonal anti-PSD-95 (6G6-1C9, Cat# NB300-556, Novus Biologicals, Centennial, CO, USA) diluted 1:1000, in 5% nonfat dry milk in TBST, and incubated overnight at 4°C using gentle shaking. .. Membranes were washed with TBST 3x 5 min each and incubated with horseradish peroxidase-conjugated anti-rabbit IgG (1:2000 dilution, Cat# 7074, Cell Signaling Technology) and anti-mouse IgG (1:2000, Novus Biologicals), for 1 h at RT in 5% non-fat dry milk in TBST.

    Gentle:

    Article Title: Low-dose dietary vorinostat increases brain histone acetylation levels and reduces oxidative stress in an Alzheimer's disease mouse model.
    Article Snippet: Nonspecific binding was blocked using 5% Blotto nonfat dry milk (Cat# sc-2325, Santa Cruz Biotechnology, Dallas, TX, USA) with 0.1% Tween-20 for 1.5 h at room temperature (RT). .. Membranes were probed with rabbit monoclonal anti-acetyl-Histone H3 (Lys9, C5B11, Cat# 9649, Cell Signaling Technology, Danvers, MA, USA) diluted 1:1000 in 5% BSA in Tris-buffered saline with 0.1% Tween-20 (TBST; 20 mM Tris·HCl (pH 7.6), 137 mM NaCl, and 0.1% (vol/vol) Tween-20), rabbit monoclonal anti-Histone H3 (D1H2, Cat# 4499, Cell Signaling Technology) diluted 1:2000, mouse monoclonal antisynaptophysin (SY38, Cat# ab8049, Abcam, Cambridge, UK) diluted 1:700, mouse monoclonal anti-PSD-95 (6G6-1C9, Cat# NB300-556, Novus Biologicals, Centennial, CO, USA) diluted 1:1000, in 5% nonfat dry milk in TBST, and incubated overnight at 4°C using gentle shaking. .. Membranes were washed with TBST 3x 5 min each and incubated with horseradish peroxidase-conjugated anti-rabbit IgG (1:2000 dilution, Cat# 7074, Cell Signaling Technology) and anti-mouse IgG (1:2000, Novus Biologicals), for 1 h at RT in 5% non-fat dry milk in TBST.

    Article Title: Low-dose dietary vorinostat increases brain histone acetylation levels and reduces oxidative stress in an Alzheimer's disease mouse model
    Article Snippet: Nonspecific binding was blocked using 5% Blotto nonfat dry milk (Cat# sc-2325, Santa Cruz Biotechnology, Dallas, TX, USA) with 0.1%Tween-20 for 1.5 h at room temperature (RT). .. Membranes were probed with rabbit monoclonal anti-acetyl-Histone H3 (Lys9, C5B11, Cat# 9649, Cell Signaling Technology, Danvers, MA, USA) diluted 1:1000 in 5% BSA in Tris-buffered saline with 0.1%Tween-20 (TBST; 20 mM Tris·HCl (pH 7.6), 137 mM NaCl, and 0.1% (vol/vol) Tween-20), rabbit monoclonal anti-Histone H3 (D1H2, Cat# 4499, Cell Signaling Technology) diluted 1:2000, mouse monoclonal anti-synaptophysin (SY38, Cat# ab8049, Abcam, Cambridge, UK) diluted 1:700, mouse monoclonal anti-PSD-95 (6G6-1C9, Cat# NB300-556, Novus Biologicals, Centennial, CO, USA) diluted 1:1000, in 5% nonfat dry milk in TBST, and incubated overnight at 4°C using gentle shaking. .. Membranes were washed with TBST 3x 5 min each and incubated with horseradish peroxidase-conjugated anti-rabbit IgG (1:2000 dilution, Cat# 7074, Cell Signaling Technology) and anti-mouse IgG (1:2000, Novus Biologicals), for 1 h at RT in 5% non-fat dry milk in TBST.



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    a , Immunoblot analysis of PARIS, pSer202/pThr205-tau (AT8), pThr181-tau (AT270), pSer396-tau, total tau, and β-actin in entorhinal cortex samples from 10-month-old mice. The location of PARIS is indicated by arrows. b–e , Quantification of total tau, AT8, AT270, and pSer396-tau levels from a . Data are mean ± SEM (PS19, n = 8; PS19; Paris -/- , n = 5). Statistical significance was assessed by two-tailed t -test. f , Immunoblot analysis of PARIS, pSer202/pThr205-tau (AT8), pThr181-tau (AT270), pSer396-tau, total tau, and β-actin in frontal cortex samples from 10-month-old mice. The location of PARIS is indicated by arrows. g–j , Quantification of total tau, AT8, AT270, and pSer396-tau levels from f . Data are mean ± SEM (PS19, n = 8; PS19; Paris -/- , n = 5). Statistical significance was assessed by two-tailed t -test. k , Representative immunofluorescence images of SYNAPSIN I (green), <t>PSD95</t> (red), and DAPI (blue) in the hippocampus of 10-month-old mice. Scale bar, 50 µm. l , m , Quantification of SYNAPSIN I–positive area and PSD95–positive area from k . Data are mean ± SEM (WT, n = 29; PS19, n = 31; PS19; Paris -/- , n = 29; images from 5–8 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. Comparisons with P ≤ 0.05 are marked on the graph.
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    a , Immunoblot analysis of PARIS, pSer202/pThr205-tau (AT8), pThr181-tau (AT270), pSer396-tau, total tau, and β-actin in entorhinal cortex samples from 10-month-old mice. The location of PARIS is indicated by arrows. b–e , Quantification of total tau, AT8, AT270, and pSer396-tau levels from a . Data are mean ± SEM (PS19, n = 8; PS19; Paris -/- , n = 5). Statistical significance was assessed by two-tailed t -test. f , Immunoblot analysis of PARIS, pSer202/pThr205-tau (AT8), pThr181-tau (AT270), pSer396-tau, total tau, and β-actin in frontal cortex samples from 10-month-old mice. The location of PARIS is indicated by arrows. g–j , Quantification of total tau, AT8, AT270, and pSer396-tau levels from f . Data are mean ± SEM (PS19, n = 8; PS19; Paris -/- , n = 5). Statistical significance was assessed by two-tailed t -test. k , Representative immunofluorescence images of SYNAPSIN I (green), PSD95 (red), and DAPI (blue) in the hippocampus of 10-month-old mice. Scale bar, 50 µm. l , m , Quantification of SYNAPSIN I–positive area and PSD95–positive area from k . Data are mean ± SEM (WT, n = 29; PS19, n = 31; PS19; Paris -/- , n = 29; images from 5–8 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. Comparisons with P ≤ 0.05 are marked on the graph.

    Journal: bioRxiv

    Article Title: Neuronal PARIS-STAT3 axis drives tau pathology and glial activation in Alzheimer’s disease

    doi: 10.64898/2025.12.06.692741

    Figure Lengend Snippet: a , Immunoblot analysis of PARIS, pSer202/pThr205-tau (AT8), pThr181-tau (AT270), pSer396-tau, total tau, and β-actin in entorhinal cortex samples from 10-month-old mice. The location of PARIS is indicated by arrows. b–e , Quantification of total tau, AT8, AT270, and pSer396-tau levels from a . Data are mean ± SEM (PS19, n = 8; PS19; Paris -/- , n = 5). Statistical significance was assessed by two-tailed t -test. f , Immunoblot analysis of PARIS, pSer202/pThr205-tau (AT8), pThr181-tau (AT270), pSer396-tau, total tau, and β-actin in frontal cortex samples from 10-month-old mice. The location of PARIS is indicated by arrows. g–j , Quantification of total tau, AT8, AT270, and pSer396-tau levels from f . Data are mean ± SEM (PS19, n = 8; PS19; Paris -/- , n = 5). Statistical significance was assessed by two-tailed t -test. k , Representative immunofluorescence images of SYNAPSIN I (green), PSD95 (red), and DAPI (blue) in the hippocampus of 10-month-old mice. Scale bar, 50 µm. l , m , Quantification of SYNAPSIN I–positive area and PSD95–positive area from k . Data are mean ± SEM (WT, n = 29; PS19, n = 31; PS19; Paris -/- , n = 29; images from 5–8 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. Comparisons with P ≤ 0.05 are marked on the graph.

    Article Snippet: Primary antibodies and working dilutions used were as follows: rabbit polyclonal anti-Synapsin I antibody (Millipore Sigma, AB1543, 1:1000); mouse monoclonal anti-PSD95 antibody (Novus Biologicals, NB300-556, 1:1000); mouse monoclonal anti-STAT3 antibody (Cell Signaling, 9139, 1:1,000); rabbit monoclonal phospho-STAT3 (Tyr705) antibody (Cell Signaling, Cat# 9145, 1:1,000); mouse monoclonal anti-glial fibrillary acidic protein (GFAP) antibody (Millipore Sigma, G3893, 1:2,000); rabbit polyclonal anti-Iba1 antibody (FUJIFILM Wako Pure Chemical Corporation, 019-19741, 1:1,000); rabbit polyclonal anti-complement C3 antibody (Invitrogen, PA5-21349, 1:1,000); rat monoclonal anti-CD16/CD32 antibody (Invitrogen, 14-0161-82, 1:1,000); mouse monoclonal anti-β-Amyloid antibody (6E10) (BioLegend, 803001, 1:1,000).

    Techniques: Western Blot, Two Tailed Test, Immunofluorescence

    a , Percentage of time in the novel arm, total distance traveled, and arm entries in the first Y-maze test. Data are mean ± SEM (Control, n = 9; CamK-PARIS, n = 9; CamK-PARIS (+Napabucasin), n = 10). Group differences were assessed by one-way ANOVA followed by Tukey’s post hoc test. b , Mean speed during Barnes maze training. Data are mean ± SEM (Control, n = 9; CamK-PARIS, n = 8; CamK-PARIS (+Napabucasin), n = 8). Group differences were assessed by one-way ANOVA followed by Tukey’s post hoc test. c , Representative immunofluorescence images of SYNAPSIN I (green), PSD95 (red), and DAPI (blue) in the hippocampus of mice. Scale bar, 50 µm. d , e , Quantification of SYNAPSIN I–positive area and PSD95–positive area from c . Data are mean ± SEM (Control, n = 33; CamK-PARIS, n = 32; CamK-PARIS (+Napabucasin), n = 31; images from 5–8 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. Comparisons with P ≤ 0.05 are marked on the graph. f , Representative co-immunostaining images of GFAP (red), C3 (green), and DAPI (blue) in the hippocampus of control and CamK-PARIS mice. Scale bar, 50 µm. g–i , Quantification of GFAP and relative C3 fluorescence from f . Data are mean ± SEM (Control, n = 38; CamK-PARIS, n = 47; CamK-PARIS (+Napabucasin), n = 51; images from 7–12 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. j , Representative co-immunostaining images of IBA1 (green), CD16/CD32 (red), and DAPI (blue) in the hippocampus of control and CamK-PARIS mice. Scale bar, 50 µm. k , l , Quantification of IBA1 and relative CD16/CD32 fluorescence from j . Data are mean ± SEM (Control, n = 43; CamK-PARIS, n = 45; CamK-PARIS (+Napabucasin), n = 50; images from 7–10 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. Comparisons with P ≤ 0.05 are marked on the graph.

    Journal: bioRxiv

    Article Title: Neuronal PARIS-STAT3 axis drives tau pathology and glial activation in Alzheimer’s disease

    doi: 10.64898/2025.12.06.692741

    Figure Lengend Snippet: a , Percentage of time in the novel arm, total distance traveled, and arm entries in the first Y-maze test. Data are mean ± SEM (Control, n = 9; CamK-PARIS, n = 9; CamK-PARIS (+Napabucasin), n = 10). Group differences were assessed by one-way ANOVA followed by Tukey’s post hoc test. b , Mean speed during Barnes maze training. Data are mean ± SEM (Control, n = 9; CamK-PARIS, n = 8; CamK-PARIS (+Napabucasin), n = 8). Group differences were assessed by one-way ANOVA followed by Tukey’s post hoc test. c , Representative immunofluorescence images of SYNAPSIN I (green), PSD95 (red), and DAPI (blue) in the hippocampus of mice. Scale bar, 50 µm. d , e , Quantification of SYNAPSIN I–positive area and PSD95–positive area from c . Data are mean ± SEM (Control, n = 33; CamK-PARIS, n = 32; CamK-PARIS (+Napabucasin), n = 31; images from 5–8 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. Comparisons with P ≤ 0.05 are marked on the graph. f , Representative co-immunostaining images of GFAP (red), C3 (green), and DAPI (blue) in the hippocampus of control and CamK-PARIS mice. Scale bar, 50 µm. g–i , Quantification of GFAP and relative C3 fluorescence from f . Data are mean ± SEM (Control, n = 38; CamK-PARIS, n = 47; CamK-PARIS (+Napabucasin), n = 51; images from 7–12 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. j , Representative co-immunostaining images of IBA1 (green), CD16/CD32 (red), and DAPI (blue) in the hippocampus of control and CamK-PARIS mice. Scale bar, 50 µm. k , l , Quantification of IBA1 and relative CD16/CD32 fluorescence from j . Data are mean ± SEM (Control, n = 43; CamK-PARIS, n = 45; CamK-PARIS (+Napabucasin), n = 50; images from 7–10 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. Comparisons with P ≤ 0.05 are marked on the graph.

    Article Snippet: Primary antibodies and working dilutions used were as follows: rabbit polyclonal anti-Synapsin I antibody (Millipore Sigma, AB1543, 1:1000); mouse monoclonal anti-PSD95 antibody (Novus Biologicals, NB300-556, 1:1000); mouse monoclonal anti-STAT3 antibody (Cell Signaling, 9139, 1:1,000); rabbit monoclonal phospho-STAT3 (Tyr705) antibody (Cell Signaling, Cat# 9145, 1:1,000); mouse monoclonal anti-glial fibrillary acidic protein (GFAP) antibody (Millipore Sigma, G3893, 1:2,000); rabbit polyclonal anti-Iba1 antibody (FUJIFILM Wako Pure Chemical Corporation, 019-19741, 1:1,000); rabbit polyclonal anti-complement C3 antibody (Invitrogen, PA5-21349, 1:1,000); rat monoclonal anti-CD16/CD32 antibody (Invitrogen, 14-0161-82, 1:1,000); mouse monoclonal anti-β-Amyloid antibody (6E10) (BioLegend, 803001, 1:1,000).

    Techniques: Control, Immunofluorescence, Immunostaining, Fluorescence